HPLC Characterization of Phytochemicals and Antioxidant Potential of Alnus nitida (Spach) Endl.

Antioxidants isolated from plants have attracted the interest of clinicians and common people to be used for systemic uses rather than synthetic antioxidants because their active role in maintaining human health with minimal side effects. Alnus nitida (Spach) Endl. is an important medicinal plant native western Himalaya widely distributed throughout Pakistan. The present study evaluates phytochemical composition this using HPLC along total content phenolics flavonoids. antioxidant activities were determined following Brand William assay. methanolic extract (Met. Ext) leaves, stem bark, seeds, roots A. scavenge free radicals such as DPPH ABTS. From fingerprinting selected portion, six possible phytochemicals confirmed. Among identified phytochemicals, there are compounds (malic acid, chlorogenic epigallocatechin gallate, quercetin, ellagic acid pyrogallol) leaves nitida, three (epigallocatechin seeds vitamin C, pyrogallol), five acid) root. Comparatively, highest potentials recorded (IC50 340 645 µg/mL against ABTS, respectively). percentages inhibition compared positive control ascorbic which produced IC50 value 60 ?g/mL each (43.81 mg GAE/g sample) found roots, while flavonoid contents (53.25 QE/g leaves. It was assumed that observed tested might due confirmed through HPLC, thus, may a valuable candidate treating oxidative stress related disorders. However, further investigations needed isolate responsible components pure from. Furthermore, toxicological effects vivo animal models also confirm results study.